Current Issue May 2012 | Vol.14 No. 3

Issue Highlights

Bile-Based Detection of Extrahepatic Cholangiocarcinoma with Quantitative DNA Methylation Markers and Its High Sensitivity
A methylation assay consisting of a five-gene panel was developed to detect extrahepatic cholangiocarcinoma in bile fluid specimens and to increase the sensitivity of preoperative diagnoses over that of bile cytology.
A New Rapid Method for Clostridium difficile DNA Extraction and Detection in Stool: Toward Point-of-Care Diagnostic Testing
A new method for the rapid diagnosis of Clostridium difficile infection, with stool sample preparation and DNA extraction in a single-use lysis microreactor followed by rapid PCR, represents an inexpensive, rapid, and reliable test for C. difficile and other infectious diseases.
Quantitative Analysis of Diagnostic Guidelines for HER2-Status Assessment
Quantitative rationales support HER2 diagnostic guidelines calling for increased numbers of evaluated cells and emphasize the importance of well-designed data analysis methods in diagnostic pathology, especially with respect to predictive clinical application.
A Method for Measuring Gene Copy Number in Biological Samples without Using Control Samples of Known Copies
A target gene and an internal control gene can be amplified by regular PCR, followed by purification and mixing at different molar ratios and real-time PCR to measure the quantification cycles (Cq) and provide target gene copy number for various applications.

Erratum
25 May 2012
In the article entitled, “Multiplex Mutation Screening by Mass Spectrometry: Evaluation of 820 Cases from a Personalized Cancer Medicine Registry” (Volume 13, pages 504–513 of the September 2011 issue...
A Novel Long-Range PCR Sequencing Method for Genetic Analysis of the Entire PKD1 Gene
17 May 2012
Ying-Cai Tan, Alber Michaeel, Jon Blumenfeld, Stephanie Donahue, Tom Parker, Daniel Levine, Hanna Rennert
Genetic testing of PKD1 and PKD2 is useful for the diagnosis and prognosis of autosomal dominant polycystic kidney disease; however, analysis is complicated by the large transcript size, the complexit...
Evaluation of Nucleic Acid Sequencing of the D1/D2 Region of the Large Subunit of the 28S rDNA and the Internal Transcribed Spacer Region Using SmartGene IDN Software for Identification of Filamentous Fungi in a Clinical Laboratory
14 May 2012
Nicole P. Kwiatkowski, Wisal M. Babiker, William G. Merz, Karen C. Carroll, Sean X. Zhang
Filamentous fungal infections have recently increased because of the increasing numbers of immunocompromised hosts. In this study, we evaluated DNA sequencing of the D1/D2 region of the large subunit ...
PCR–Electrospray Ionization Mass Spectrometry: The Potential to Change Infectious Disease Diagnostics in Clinical and Public Health Laboratories
14 May 2012
Donna M. Wolk, Erin J. Kaleta, Vicki H. Wysocki
During the past 20 years, microbial detection methods have included those that are genetic based, such as real-time PCR and peptide nucleic acid fluorescent hybridization, which coexist with tradition...
Multicenter Evaluation of the LightCycler MRSA Advanced Test, the Xpert MRSA Assay, and MRSASelect Directly Plated Culture with Simulated Workflow Comparison for the Detection of Methicillin-Resistant Staphylococcus aureus in Nasal Swabs
14 May 2012
Rodney C. Arcenas, Stacey Spadoni, Amin Mohammad, Frederick L. Kiechle, Kimberly Walker, Robert C. Fader, Francoise Perdreau-Remington, John Osiecki, Oliver Liesenfeld, Shelby Hendrickson, Arundhati Rao
Rapid detection of nasal colonization with methicillin-resistant Staphylococcus aureus (MRSA) followed by appropriate infection control procedures reduces MRSA infection and transmission. We compared ...

View More Articles in Press...

Relevance, Pathogenesis, and Testing Algorithm for Mismatch Repair–Defective Colorectal Carcinomas: A Report of the Association for Molecular Pathology
March 2012 (Vol. 14 | No. 2 | Pages 91-103)
William K. Funkhouser, Ira M. Lubin, Federico A. Monzon, Barbara A. Zehnbauer, James P. Evans, Shuji Ogino, Jan A. Nowak
Plasma Components Affect Accuracy of Circulating Cancer-Related MicroRNA Quantitation
January 2012 (Vol. 14 | No. 1 | Pages 71-80)
Dong-Ja Kim, Sarah Linnstaedt, Jaime Palma, Joon Cheol Park, Evangelos Ntrivalas, Joanne Y.H. Kwak-Kim, Alice Gilman-Sachs, Kenneth Beaman, Michelle L. Hastings, Jeffrey N. Martin, Dominik M. Duelli
Next-Generation Sequencing for Simultaneous Determination of Human Papillomavirus Load, Subtype, and Associated Genomic Copy Number Changes in Tumors
March 2012 (Vol. 14 | No. 2 | Pages 104-111)
Caroline Conway, Rebecca Chalkley, Alec High, Kenneth Maclennan, Stefano Berri, Preetha Chengot, Melissa Alsop, Philip Egan, Joanne Morgan, Graham R. Taylor, John Chester, Mehmet Sen, Pamela Rabbitts, Henry M. Wood
Technical Validation of a Tm Biosciences Luminex-Based Multiplex Assay for Detecting the American College of Medical Genetics Recommended Cystic Fibrosis Mutation Panel
July 2006 (Vol. 8 | No. 3 | Pages 371-375)
Charles M. Strom, Richard Janeszco, Franklin Quan, Sheng-biao Wang, Arlene Buller, Matthew McGinniss, Weimin Sun
Guidance for Fluorescence in Situ Hybridization Testing in Hematologic Disorders
April 2007 (Vol. 9 | No. 2 | Pages 134-143)
Daynna J. Wolff, Adam Bagg, Linda D. Cooley, Gordon W. Dewald, Betsy A. Hirsch, Peter B. Jacky, Kathleen W. Rao, P. Nagesh Rao, the Association for Molecular Pathology Clinical Practice Committee and the American College of Medical Genetics Laboratory Quality Assurance Committee

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Current impact factor: 4.219 (2010 JCR)
Current 5-year impact factor: 4.059 (2010 JCR)
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About JMD

The Journal of Molecular Diagnostics, co-published by the Association for Molecular Pathology (AMP) and the American Society for Investigative Pathology (ASIP), publishes high quality original papers on scientific advances in the translation and validation of molecular discoveries in medicine into the clinical diagnostic setting, and the description and application of technological advances in the field of molecular diagnostic medicine.

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The Association for Molecular Pathology (AMP) was founded in 1995 to provide structure and leadership to the then-emerging field of molecular diagnostics. Since its onset, AMP has worked to develop mechanisms for training and certification in diagnostic molecular pathology and to shape regulations and policy that influence research and the practice of molecular diagnostics. The organization is divided into the scientific subdivisions of genetics, infectious diseases, hematopathology, and solid tumors. The AMP membership includes individuals from academia, government, and industry, including basic scientists, laboratory directors, medical technologists, and trainees.

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The Journal of Molecular Diagnostics is published by Elsevier for the Association for Molecular Pathology (AMP) and the American Society for Investigative Pathology (ASIP).